Figure 7.
Analysis of crossover interference in haploids using snATAC-seq data. The distance of observed crossovers was compared with the null hypothesis generated from permutation via label swapping to simulate the absence of crossover interference. (A) The median distance for observed double crossover chromosomes from wild-type samples was ∼88.8 Mb, compared with 44.0 Mb in the null hypothesis. Pairwise comparisons using Wilcoxon rank sum test with continuity correction, P= 2 × 10−16. (B) The median distance for observed double crossover chromosomes from mutant samples was ∼82.6 Mb, compared with 46.1 Mb in the null hypothesis. Pairwise comparisons using Wilcoxon rank sum test with continuity correction, P< 2 × 10−16. (C). Median intercrossover distances were reduced by 6.2 Mb in mutant crossovers, with an interquartile range of 5.9 Mb. These findings suggest that the additional crossovers in Fancm-deficient mice are likely to be derived from the non-interfering (type II) crossover pathway. Pairwise comparisons using Wilcoxon rank sum test with continuity correction, P< 2 × 10−7. Non-parametric statistical testing was used due to non-normal data distribution.