TABLE 2.
Biochemical characterization of strain AKE1
| Strain | Growth conditionsa | Sp act (U/g)b
|
|||
|---|---|---|---|---|---|
| NSDO | DHNDO | HBPA | SalDH | ||
| AKE1 | Glucose | 3.2 | 0 | 25 | 82 |
| Glucose + salicyclate | 7.7 | 0 | 158 | 142 | |
| BN6wt | Glucose | 1.4 | 0 | 31 | 84 |
| Glucose + salicyclate | 7.3 | 430 | 156 | 111 | |
a
Cell suspensions of strain AKE1 (ΔnsaC::neo) and BN6wt were grown exponentially in MMG or in MMG supplemented with 0.5 mM salicylate for 3 h.
b
Specific enzyme activities for the catabolism of 2NS in strain BN6wt were determined as described in Materials and Methods. Specific activity of the ring-hydroxylating dioxygenase NSDO was measured with resting cells by using naphthalene-2-carboxylic acid as the substrate (35). HBPA, 2′-hydroxybenzalpyruvate aldolase; SalDH, salicylaldehyde dehydrogenase.