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Deletion analysis of the 7.0-kb EcoRI fragment. The direction of transcription from the lac promoter in a vector is depicted by a thin arrow. The 5CVA decarboxylase activity of E. coli containing each plasmid is presented on the right. Abbreviations: A, ApaI; B, BamHI; Bt, BstXI; E, EcoRI; H, HindIII; N, NotI; P, PstI; S, SalI; Sc, SacI; X, XhoI. ND, not detected.
