FIG. 6.

Localization of the lignin degradation genes in the genome of S. paucimobilis SYK-6. PFGE profiles and a summary of the lig gene localizations are indicated in panel A. Lane 1, SpeI digests of the SYK-6 total DNA; lane 2, AseI digests of the 1-Mb SpeI fragment shown in lane 1; lanes 3 and 4, lambda ladder markers; lane 5, AflII digests of the 1-Mb SpeI digest. The restriction maps of pCVA, pLE6, and pTE01 containing ligW, ligX, and ligZ-ligY, respectively, are presented in panel B. The large arrows indicate the coding region of each gene. The restriction map of the region shown by a dashed line in pCVA was not determined. Abbreviations: A, ApaI; B, BamHI; BII, BglII; E, EcoRI; EV, EcoRV; H, HindIII; N, NotI; P, PstI; S, SalI; Sc, SacI; X, XhoI.