TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| S. paucimobilis | ||
| SYK-6 | Wild type; Nalr Smr | 13 |
| AKB | Mutant derivative of SYK-6; ligB::Kmr; Nalr Smr Kmr | H. Aoshima et al., ISME-8, 1998 |
| P. putida PpY101 | Nalr Smr | 9 |
| E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-proAB) F′[traD36 proAB+lacIqlacZΔM15] | 36 |
| E. coli HB101 | supE44 hsdS20(rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | 3 |
| Plasmids | ||
| pVK100 | Broad-host-range cosmid vector, Kmr Tetr | 6 |
| pRK2013 | Kmr Tra+ Mob+, ColE1 replicon | 7 |
| pKT230 | Broad-host-range vector; Kmr | 2 |
| pBluescript II KS(+) | Cloning vector; ColE1 replicon; Apr | 32 |
| pUC18 and pUC19 | Cloning vectors; ColE1 replicon; Apr | 36 |
| pCVA | pVK100 with an approx 20-kb DNA fragment carrying ligW | This study |
| pVK7E2 | pVK100 with a 7.0-kb EcoRI fragment of pCVA | This study |
| pKS7E2 | KS(+) with a 7.0-kb EcoRI fragment of pVK7E2 | This study |
| pKS7E1 | KS(+) carrying the same fragment as pKS7E2 but in the opposite direction | This study |
| pKV6, pKV9, pKV21 | Deletion derivatives of pKS7E2 | This study |
| p1N2 | KS(+) with a 3.3-kb NotI-EcoRI fragment of pKS7E2 | This study |
| pAH34, pAH47 | Deletion derivatives of p1N2 | This study |
| p1SC2 | KS(+) with a 2.4-kb SacI-EcoRI fragment of pKS7E2 | This study |
| pTE01 | pUC19 with a 15-kb EcoRI fragment carrying ligZ and ligY | 26 |
| pLE6 | pKT230 with a 6.0-kb EcoRI fragment carrying ligX | 33 |
| pHN139F | pUC18 with a 10.5-kb EcoRI fragment carrying ligJAB, a part of ligC, and ligI | 22 |
| pBE10 | pVK100 with an approx 24-kb DNA fragment carrying ligDFE | 17 |