TABLE 2.
Sequences of PCR primers used for amplification of the pcpB gene
| Primer | Sequence | Direction | Target sitea | Source or reference |
|---|---|---|---|---|
| PcpB-G | 5′-GGSTTCACSTTCAAYTTCGA-3′ | Forward | 262-281 | 1 |
| PcpB-D2 | 5′-TCCTGCATSCCSACRTTCAT-3′ | Reverse | 946-965 | 1 |
| PcpB-D3 | 5′-ACGTTGCATCGAGATGCTG-3 | Reverse | 382-400 | This study |
| PcpB-E2 | 5′-CGACTGGATTCACTATTTCAT-3′ | Forward | 646-666 | This study |
| PcpB-E3 | 5′-CGCTGGCGACCGCGTATC-3′ | Forward | 1259-1276 | This study |
| PcpB-F1 | 5′-CTGCAGTTACACTAACAATG-3′ | Forward | −35-−16 | This study |
| PcpB-F2 | 5′-CGACGTCGATCATATTCG-3′ | Reverse | 1652-1669 | This study |
a
Corresponds to the nucleotide numbering from the beginning of the open reading frame of the Novosphingobium sp. strain MT1 pcpB gene (AJ319678).