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. 2002 Sep;68(9):4201–4208. doi: 10.1128/AEM.68.9.4201-4208.2002

TABLE 1.

Primers used in PCR experiments

Primer Positiona Sequence 5′→3′ Annealing temp (°C) Refer- ence(s)
F536 519-536 CAGCAGCCGCGGTAATAC 62 32
R1406 1406-1389 ACGGGCGGTGTGTACAAG 62 32
F67 50-67 AACACATGCAAGTCGAAC 55 32
R685 685-666 TCTACGCATTTCAC(CT)GCTAC 55 32
F984 968-984 AACGCGAAGAACCTTAC 53 15, 17
F984GC 968-984 CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTAC 53 15
R1378 1401-1378 CGGTGTGTACAAGGCCCGGGAACG 53, 63 15, 17
F243 226-243 GGATGAGCCCGCGGCCTA 63 15
F203α 175-203 CCGCATACGCCCTACGGGGGAAAGATTTAT 56 13
F948β 931-948 CGCACAAGCGGTGGATGA 64 13
L 1514-1494 CTACGG(AG)TACCTTGTTACGAC 56, 64 K. Smalla
a

Escherichia coli numbering (4).