TABLE 1.
Primer | Positiona | Sequence 5′→3′ | Annealing temp (°C) | Refer- ence(s) |
---|---|---|---|---|
F536 | 519-536 | CAGCAGCCGCGGTAATAC | 62 | 32 |
R1406 | 1406-1389 | ACGGGCGGTGTGTACAAG | 62 | 32 |
F67 | 50-67 | AACACATGCAAGTCGAAC | 55 | 32 |
R685 | 685-666 | TCTACGCATTTCAC(CT)GCTAC | 55 | 32 |
F984 | 968-984 | AACGCGAAGAACCTTAC | 53 | 15, 17 |
F984GC | 968-984 | CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGGAACGCGAAGAACCTTAC | 53 | 15 |
R1378 | 1401-1378 | CGGTGTGTACAAGGCCCGGGAACG | 53, 63 | 15, 17 |
F243 | 226-243 | GGATGAGCCCGCGGCCTA | 63 | 15 |
F203α | 175-203 | CCGCATACGCCCTACGGGGGAAAGATTTAT | 56 | 13 |
F948β | 931-948 | CGCACAAGCGGTGGATGA | 64 | 13 |
L | 1514-1494 | CTACGG(AG)TACCTTGTTACGAC | 56, 64 | K. Smalla |
Escherichia coli numbering (4).