Gel electrophoresis of PCR products carried out on genomic DNAs of different Leptosphaeria isolates with primer pairs A1-A3, A2-A3, and NA1-3-NA2-1. Lanes 1 and 2, L. maculans aggressive isolate; lanes 3 and 4, L. maculans nonaggressive isolate; lane 5, Leptosphaeria sp. isolated from S. castanea spores.