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Effect of cytochrome c (Cyt. c) on the formation of H2O2 during oxidation of NADH (250 μM) by NADH oxidase in CE of anaerobically grown cells as measured with an oxygen electrode. Panels: A, assay without initial addition of cytochrome c; B, assay with initial addition of cytochrome c. After complete oxidation of NADH, catalase was added to the assay to detect H2O2.
