FIG. 5.

Effect of sodium salicylate on E. coli major porin expression and on S1-GFP expression. Immunodetection was performed by using OmpF (A), OmpC (B), and GFP (C) antibodies. TG1-GFP and TG1-S1-GFP were cultivated in LB until the A₆₀₀ reached 0.2, and 5 mM sodium salicylate was added. Samples were collected at different times, as follows: lanes 1, controls; lanes 2, 1 h; lanes 3, 2 h; lanes 4, 3 h.