Figure 7.

Transcription induction of cta3⁺ in cells lacking Tup11 and Tup12 is Atf1 dependent. (A) Strains used are indicated above the lanes and were wild type (w.t.) (NT4), atf1Δ (NT147), tup11Δ tup12Δ (SW76), and atf1Δ tup11Δ tup12Δ (SW92). Log phase cultures growing at 30°C in YE5S (lanes 1, 3, 5, and 7) were incubated with KCl (to a final concentration of 0.6 M) for 15 min (lanes 2, 4, 6, and 8). Total RNA was extracted, separated by electrophoresis and subjected to Northern analysis with the indicated probes. The level of cdc2⁺ mRNA was used as a loading control. (B) Quantification of cta3⁺ mRNA levels in A. (C) As for A, except strains used were wild type (w.t.) (NT4), pcr1Δ (JX25), tup11Δ tup12Δ (SW76), and pcr1Δ tup11Δ tup12Δ (RJP59). (D) Quantification of cta3⁺ mRNA levels in C.