Bud8p is localized to the distal tip of haploid cells, and its localization is dependent upon Bud6p and Bni1p. Strain backgrounds (wild-type, SY3695; pea2, SY3698; bud6, SY3696; and bni1, SY3697) are as shown. Growth on SCD (+Glu) or SC medium (−Glu) is as indicated. Left, DIC. Right, FITC filter. All pictures were taken at the same scale; bar, 5 μm.