Figure 3.

Bud8p is required for localization of Bud2p and actin to the distal pole in glucose-limiting conditions. Left four panels, GFP-Bud2p localization. bud2 (top, SY3707) and bud2 bud8 (bottom, SY3708) strains containing a GFP-BUD2 fusion plasmid were grown in glucose-limiting conditions. Cells were photographed using DIC (left) or FITC filters (right). Right four panels, actin localization. Wild-type (SY3687) and bud8 mutant (SY3689) cells grown in glucose-limiting conditions were stained with rhodamine-phalloidin and photographed using DIC (left) or Rh filters (right). Arrows indicate regions of highly localized actin patches. All pictures were taken at the same scale; bar, 5 μm.