Diploid cells exhibit filamentous growth in response to limiting glucose. (A) Single cell assay. Diploid cells (SY3734) were spread onto SC (−Glu) and SCD (+Glu) medium, incubated for 16 h at 25°C, and photographed. Bars, 10 μm. (B) Equal concentrations of diploid cells (SY3734) and haploid cells (SY3687) were spotted onto YPD medium and grown for 2 d at 30°C. Plates were photographed (left), washed, and photographed again (right).