Table 4.
Budding patterns of strains deleted for AXL1 and/or BUD8 or overexpressing AXL1
| Genotype | Distal | Equatorial | Proximal |
|---|---|---|---|
| Wild-type (+Glu)a | 4 | 1 | 95 |
| axl1 (+Glu) | 55 | 4 | 41 |
| axl1 bud8 (+Glu) | 16 | 37 | 47 |
| Wild-type (−Glu)b | 69 | 1 | 30 |
| axl1 (−Glu) | 82 | 10 | 8 |
| axl1 bud8 (−Glu) | 25 | 9 | 66 |
| bud8 (−Glu) | 10 | 1 | 89 |
| Wild type (YPGal)c | 30 | 0 | 70 |
| GAL1-AXL1 (YPGal) | 12 | 1 | 87 |
a
For +Glu, cells were grown in YPD to mid-log phase, and bud scars were stained and counted as described in MATERIALS AND METHODS. Strains are as indicated: wild type (SY3687), axl1 (SY3721), axl1 bud8 (SY3722), bud8 (SY3689), and GAL-AXL1 (SY3723).
b
For −Glu, invaded cells were collected, stained, and counted as described in MATERIALS AND METHODS.
c
Cells were spotted onto solid agar YPGal medium for 24 h and prepared as for the −Glu experiments (see footnote b).