Table 5.
Bud position of the rsr1 mutant grown on glucose-rich or glucose-limited medium
| Genotype | Bud | Distal | Equatorial | Proximal |
|---|---|---|---|---|
| Wild type (SC)a | 1st | 97 | 0 | 3 |
| rsr1 (SC) | 1st | 95 | 2.5 | 2.5 |
| Wild type (SC) | 2nd | 88 | 2 | 10 |
| rsr1 (SC) | 2nd | 29 | 35.5 | 35.5 |
| rsr1 bud8 (SC) | 1st | 95 | 2 | 3 |
| Wild type (SCD) | 1st | 5 | 0 | 95 |
| rsr1 (SCD) | 1st | 57 | 29 | 14 |
| grr1 (SCD) | 1st | 14 | 0 | 86 |
| rsr1 grr1 (SCD) | 1st | 85 | 11 | 4 |
a
Strains are as indicated: wild type (SY3687), rsr1 (SY3724), rsr1 bud8 (SY3725), grr1 (SY3726), and rsr1 grr1 (SY3727). Microcolonies of <10 cells were examined for bud placement by microscopy, and all observed microcolonies were included in the analysis. Bud position was considered distal if the bud was observed in the distal third of the cell; equatorial, for the middle third of the cell; and proximal, for the proximal third of the cell. For each sample, >200 buds were counted, of which <20 were ambiguous; these were not included in the final percentage. Analysis was performed on glucose-limited (SC) or glucose-rich (SCD) medium.