Table 2.
The primer sequences used for quantitative real-time PCR analysis in Nile tilapia.
| Gene | Forward primer (5'- to 3'-) |
Reverse primer (5'- to 3'-) |
Amplification efficiency (%) | Accession no. |
|---|---|---|---|---|
| grp78 | GCAAGCCCCATATCCAGGTT | CCAGGTAAGCCTCAGCAGTC | 94 | XM_019357245.1 |
| caspase 3 | GGAGTGGACGATACAGACGCAAA | TGAAGCTGTGTGACTGGGGCTT | 93 | NM_001282894.1 |
| caspase 8 | AACTGGAATCTTCTGAGGTGGCA | TTGAGAAGAGATCTTTGGCCTGC | 95 | XM_005475926.3 |
| caspase 9 | ATACTTGAGGAAAACGCTGCCACT | GAACCAGGCATTTGTTTGTAGAGC | 96 | XM_003455320.4 |
| bax | GGAGGAGGCGATCAAGGGAAT | TTTTTGCCTCTGAACTCGCTCA | 99 | XM_019357746.1 |
| bcl2 | ATCGCAGACTGGATGACGGAGTAT | TCTGTCTGTCGTACAGCTCCACAA | 97 | XM_003437902.4 |
| ire | TGTAAGAAGCTCGCAGTGGG | TTGTCTTTGCAGTCCTCGCT | 95 | XM_005461083.3 |
| crt | GATGCCAAGAAGCCCGATGA | GGCAGGGTTGTCGATCTCTC | 102 | XM_005478351.4 |
| perk | GCAGAGTCCAGCGTTTACCT | CGATGGCTTTGGAGGGGAAT | 94 | XM_003447769.4 |
| xbp1s | GCCTTTCCTGTTACGGAGGTTGTG | GCCAGGCAGGCTTCTTTCTCC | 98 | XM_031746876.2 |
| chop | TACATGCACCGAGAAGGAGC | GACGAGTTGTGATGCAGGGT | 104 | XM_003451826.5 |
| il-1β | GAGCACAGAATTCCAGGATGAAAG | TGAACTGAGGTGGTCCAGCTGT | 95 | XM_019365841.1 |
| tgf-β1 | AAGAGGAGGAGGAATACTTTGCCA | GAAGCTCATTGAGATGACTTTGGG | 96 | NM_001311325.1 |
| tnf-α | CAGAAGCACTAAAGGCGAAGAACA | TTCTAGATGGATGGCTGCCTTG | 94 | NM_001279533 |
| mif | AGCAGAAGCAGGAAGGCGAAGA | CGGTACATCACCTCTGGCAACATT | 102 | XM_003444573.3 |
| il-12 | AGGTCAGCCAATCTGTGCCACT | CCGTGATGTTCTGGAGCAGTGTTC | 95 | XM_003437924.4 |
| cox-2 | TGCTGAAAGAGGTCCACCCATACT | CGCTCAGATGCTGCACGTAGTC | 99 | XM_031758642.2 |
| β-Actin | AGCCTTCCTTCCTTGGTATGGAAT | TGTTGGCGTACAGGTCCTTACG | 94 | KJ126772.1 |
| 18s | CCGGGTATGCGTGCATTTAT | CTGATTCCCCGTTACCCGTG | 103 | XR_003216134.1 |
Note: bax, bcl2-associated x protein; 18s, 18s ribosomal RNA; xbp1s, spliced x-box binding protein 1.
Abbreviations: β-actin, beta-actin; bcl2, b-cell lymphoma 2; caspase 3, cysteine-aspartic protease 3; caspase 8, cysteine-aspartic protease 8; caspase 9, cysteine-aspartic protease 9; chop, c/ebp homologous protein; cox-2, cyclooxygenase-2; crt, calreticulin; grp78, glucose-regulated protein 78; il-1β, interleukin-1 beta; il-12, interleukin-12; ire, inositol-requiring enzyme; mif, macrophage migration inhibitory factor; perk, protein kinase r-like endoplasmic reticulum kinase; tgf-β1, transforming growth factor beta 1; tnf-α, tumor necrosis factor alpha.