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. 2002 Sep;13(9):3029–3041. doi: 10.1091/mbc.E02-04-0203

Figure 5.

Figure 5

Chromosome missegregation associated with genomic alleles of BUB1. (A) Colony sectoring phenotypes of bub1-E333K (bub1-1) and bub1-K733R. Strains were YCD280 and YCD281. (B) Chromosome loss caused by genomic alleles. Left: Half sector analysis was used to analyze the mutants as shown. WT: 8/8,503. bub1-E333K: 248/5,964. bub1-K733R: 83/6,576. bub1[1-210]: 354/6,769. bub1[1-367]: 477/9,019. bub1[1-608]: 222/14,105. bub1[211-1021]: 355/14,837. Strains were YCD279, YCD280, YCD281, YCD371, YCD358, and YCD407. Right: A centromere plasmid containing a MET25-inducible BUB3 gene (pBUB3) or vector alone (p415MET) was introduced into wild-type (WT, YPH278), bub1-E333K (YCD280), or bub1-K733R (YCD281) strains. Half sector analysis was performed after plating on methionine-free media. WT + p415MET: 1/3,728. WT + pBUB3: 3/4,925. bub1-E333K + p415MET: 104/1,567. bub1-E333K + pBUB3: 48/3,941. bub1-K733R + p415MET: 53/3,654. bub1-K733R + pBUB3: 36/4,102.