Figure 1.
Expression of TGF-β superfamily signaling components in HPAECs and PASMCs. RNA samples from HPAECs and PASMCs were analyzed by RT-PCR for expression of TGF-β superfamily-signaling components and the housekeeping gene β-actin. Two alternatively spliced forms, WT and SH, of BMPR-II mRNA transcripts were detected. As controls, RNAs from HPAECs and PASMCs were analyzed for β-actin expression without the prior generation of cDNA, and a PCR reaction for each set of primers was run against H2O.