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Hetero-oligomerization of FLAG-tagged BMPR-II with HA-tagged ALK-3 in transfected COS-7 cells. Top, cell lysates were immunoprecipitated with anti-FLAG antibody followed by immunoblotting with anti-HA antibody. Expression of ALK-3 (middle) and BMPR-II (bottom) was confirmed by immunoblotting of cell lysates with anti-FLAG and anti-HA antibodies, respectively. Fast- and slowly migrating bands of BMPR-II and ALK-3 are indicated by open and closed triangles, respectively.
