Table 1.
Plasmids used in this study
| Plasmid name | Construct | Source | If PCR, Oligos Used |
|---|---|---|---|
| pAB169 | pGAD-GGA1 · N | Boman et al., 2000 | |
| pAB202 | pBG4D-hARF3(Q71L) | Boman et al., 2000 | |
| pAB318 | pcDNA3-HA-hGGA1ΔVHS | Boman et al., 2000 | |
| pAB348 | pcDNA3-HA-hGGA1 | Boman et al., 2000 | 797 and 701 |
| pAB354 | pACT2-hGGA3 | Boman et al., 2000 | |
| pAB361 | pACT2-Ggalp(1–330) | Zhdankina et al., 2001 | |
| pAB362 | pACT2-Ggalp(1–330, L203Q) | This study | |
| pAB363 | pcDNA3-HA-hGGA3 | Boman et al., 2000 | 795 and 780 |
| pAB374 | pACT2-Gga2p(1–326) | Zhdankina et al., 2001 | |
| pAB382 | pGEX5X-Gga2p(1–326) | Zhdankina et al., 2001 | |
| pAB418 | pACT2-hGGA1ΔN(L182Q) | This study | A025 and A026, 638 and A028 |
| pAB441 | pRS316-GGA2 | Zhdankina et al., 2001 | A037 and A038 |
| pAB443 | pcDNA3-HA-hGGA1(L182Q) | This study | A025 and A026, 797 and A028 |
| pAB454 | pBG4D-yArf2(Q71L) | Zhdankina et al., 2001 | |
| pAB456 | pRS316-GGA2(I207N) | This study | A044 and A045, A037 and A038 |
| pAB462 | pACT2-Gga2p(1–326, I207N) | This study | |
| pAB468 | pRS316-GGA2(1–326) | This study | A037 and A049 |
| pAB469 | pRS316-GGA1(L203Q) | This study | |
| pAB470 | pRS316-GGA1 | Zhdankina et al., 2001 | A041 and A048 |
| PAB472 | pGEX5X-Gga2p(1–326, I207N) | This study | |
| pAB477 | pRS316-GGA2(1–326, I207N) | This study | A037 and A049 |
| pAB493 | pRS315-GGA2(PEDL→AAAL) | This study | USE mutagenesis, A056/A060 |
| pAB503 | pACT2-hGGA3(L149Q) | This study | A092 and A093, 772 and 637 |
| pAB504 | pGFP-Gga2p(I207N) | This study | A097 and A098 |
| pAB505 | pGFP-Gga1p(L203Q) | This study | |
| pAB506 | pCDNA3-HA-hGGA3(L149Q) | This study | |
| pAB509 | pRS315-GGA2(ANKL→AAAL) | This study | USE mutagenesis, A056/A061 |
| pAB510 | pGFP-Gga1p(141–331) | This study | A107 and A108 |
| pAB511 | pGFP-Gga1p(141–331, L203Q) | This study | A107 and A108 |
| pAB512 | pGFP-Gga1p(140–557) | This study | A107 and T7 |
| pAB513 | pGFP-Gga1p(140–557, L203Q) | This study | A107 and T7 |
| pAB524 | pGFP-Gga1p(1–331) | This study | A024 and A108 |
| pAB525 | pGFP-Gga1p(1–331, L203Q) | This study | A024 and A108 |
| pAB526 | pGFP-Gga1p(1–133) | This study | |
| pAB529 | pRS316-GGA2(PEDL→AAAL) | This study | |
| pAB530 | pRS316-GGA2(ANKL→AAAL) | This study | |
| pAB532 | pGFP-Gga1p(326–557) | This study | A118 and T7 |
| pAB533 | pGFP-Gga1p(421–557) | This study | A119 and T7 |
| pAB543 | pGFP-Gga1p(140–430) | This study | A107 and A121 |
| pAB555 | pRS316-GGA2 (110–585) | This study | |
| pAB580 | pACT2-Gga2pANKL→AAAL | This study | |
| pAB581 | pACT2-Gga2pPEDL→AAAL | This study | |
| pAB582 | pcDNA3-hGGA1ΔVHS(L182Q) | This study | |
| pCKR3A | vps1∷LEU2 in pUC12HP | Rob Piper | |
| pCS135 | pGFP-Gga1p, URA3 2μ | Scott Emr | |
| pCS136 | pGFP-Gga2p, URA3 2μ | Scott Emr | |
| pKJH2 | vps27∷LEU2 in pUC19 | Piper et al., 1995 | |
| pMR2654 | 3HA as XbaI fragment | Kurihara et al., 1996 | |
| pNB1092 | RFP-GYP1 in pRS316 | (Du and Novick, 2001) |