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dnf1,2,3Δ mutant exhibits a defect in Snc1p-GFP recycling. (A) pRS416 SNC1-GFP was introduced into BY4742 (WT), PFY3273A (dnf1,2,3Δ) and ZHY615D1C (drs2Δ) strains. Cells were grown at 30°C to log phase and examined by fluorescence microscopy. (B) Cells harboring pRS426 STE2-GFP were grown at 30°C to log phase and examined by fluorescence microscopy. MetaMorph 4.5 was used to process the image and measure the fluorescence intensity on cell surface and in the vacuoles.
