Table 4.
Sporulation defect and CW sensitivity of drs2/dnf deletion mutants
| Strains | Sporulation defect | Calcofluor white sensitivity |
|---|---|---|
| Wild type | − | − |
| drs2Δ | + | ++ |
| dnf1Δ | − | − |
| dnf2Δ | − | − |
| dnf3Δ | − | − |
| dnf1Δ dnf2Δ | + | ++ |
| dnf1Δ dnf3Δ | +/− | − |
| dnf2Δ dnf3Δ | − | − |
| dnf1Δ dnf2Δ dnf3Δ | + | +++ |
Sporulation was assessed in homozygous diploids, whereas the Calcofluor white sensitivity was examined in haploid strains as described in MATERIALS AND METHODS. Sporulation defect: −, no significant sporulation defect; +, complete sporulation defect; +/−, sporulation efficiency is ∼0.5% of wild type. Calcofluor white sensitivity: growth on 50 μg/ml CW (−); no growth on 30 μg/ml CW plates (++); no growth on 10 μg/ml CW plates (+++).