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. 2002 Sep;13(9):3192–3202. doi: 10.1091/mbc.E02-04-0216

Figure 4.

Figure 4

The expression of a dominant-negative mutant of SMN (SMNΔN27) perturbs the nuclear localization of hTERT. (A) HeLa cells were transiently cotransfected with DNA constructs expressing either Myc-SMN and YFP-SmB (a–d) or Myc-SMNΔN27 and YFP-SmB (e–h). The fixed and permeabilized cells were stained for Myc-SMN (c) and Myc-SMNΔN27 (g) using anti-Myc. DNA stained with DAPI shows the nucleus of each cell (a and e). Images b and c, and f and g, are merged to form d and h, respectively. (B) HeLa cells were transiently cotransfected with DNA constructs expressing either Myc-SMN and GFP-hTERT (a–d) or Myc-SMNΔN27 and GFP-hTERT (e–l). The fixed and permeabilized cells were stained for Myc-SMN (c) and Myc-SMNΔN27 (g and k) using anti-Myc. DNA stained with DAPI shows the nucleus of each cell (a, e, and i). Images b and c, f and g, and j and k are merged to form d, h, and l, respectively. The arrows point to the nuclear gems and the arrowheads to the SMNΔN27-induced cytoplasmic accumulations. Bar, 12 μm.