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. 2002 Sep;13(9):3218–3234. doi: 10.1091/mbc.E02-03-0134

Figure 7.

Figure 7

Coordinate regulation of JAM and ICAM-1 in response to TNF-α and IFN-γ exposure. (A) JAM mRNA expression as determined by cQRT-PCR with an internal standard (ΔJAM) at the indicated concentration (1 to 0.0001 ng). Left, Vehicle, and right, cultures exposed to TNF-α and IFN-γ for 72 h. (B) Semiquantitative RT-PCR (QRT-PCR) analysis of ICAM-1 mRNA expression normalized to GAPDH. Top, ICAM-1 expression in vehicle or at 24, 48, or 72 h after exposure to TNF-α and IFN-γ. Bottom, GAPDH expression. (C, D) JAM (C) and ICAM-1 (D) protein expression determined by ELISA at 24, 48, and 72 h after exposure to TNF-α and IFN-γ. Data are from a minimum of n = 6 cultures from at least three patients.