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Association of kendrin with γ-tubulin (γTub) through binding with GCP2. COS7 cells were transfected with various combinations of expression plasmids as shown at the top. Then the cell extracts (Extr) were immunoprecipitated with anti-FLAG (αFL) or control (Ctr) mouse IgG followed by immunoblot with anti-Myc for γ-tubulin (top), anti-HA for GCP2 or GCP3 (middle), or anti-FLAG for kendrin1–1189 (bottom).
