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. 2002 Sep;13(9):3235–3245. doi: 10.1091/mbc.E02-02-0112

Figure 7.

Figure 7

Association of endogenous kendrin, CG-NAP, γ-tubulin, and GCP2. (A) Association of endogenous CG-NAP and kendrin. HeLa cell extracts (Extr) were immunoprecipitated with anti-CG-NAP (αEE+αBH) or anti-kendrin (αKen) or control (Ctr) rabbit IgG followed by immunoblot with anti-CG-NAP (αEE) (top) or αKen (bottom). (B) Association of recombinant kendrin with CG-NAP. 293T cells were cotransfected with FLAG-tagged (FL) CG-NAP and HA-tagged kendrin. Then the cell extracts were immunoprecipitated with anti-FLAG or control mouse IgG followed by immunoblot with anti-FLAG (lanes 1–3) or anti-HA (lanes 4–6). (C) Specificity of anti-GCP2 antibody. The extracts of COS7 cells transfected with HA-tagged GCP2 or HeLa cells were immunoprecipitated with αGCP2 or control rabbit IgG followed by immunoblot with αGCP2 (lanes 1–3) or anti-HA (lanes 4–6). (D) Association of GCP2 with CG-NAP and kendrin as well as with γ-tubulin. HeLa cell extracts were immunoprecipitated with αGCP2 or control rabbit IgG followed by immunoblot with anti-CG-NAP (αEE), αKen, αGCP2, or anti-γ-tubulin.