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. Author manuscript; available in PMC: 2025 Sep 9.
Published in final edited form as: Methods Mol Biol. 2023;2652:147–169. doi: 10.1007/978-1-0716-3147-8_8

Fig. 3.

Fig. 3

(a) Flow chart comparing conventional purification method and heat treatment method. (b) SDS-PAGE of purification of CD2 (6 kDa) using heat treatment method (lanes-3–4) or conventional purification method (lanes-5–6): lane-1, pre-stained protein marker; lane-2, cleaved GST-CD2 using thrombin; lane-3, pellet after heat treatment; lane-4, supernatant after heat treatment; lane-5, eluted CD2 in flow-through; lane-6, eluted GST with 10 mM reduced GSH. (c) Western blot of heat treatment method: lane-1, GST-CD2; lane-2, cleaved GST-CD2; lane-3, supernatant post-heat treatment, lane-4, pellet post-heat treatment; lane-5, pre-stained protein marker. (d) Yields of CD2: heat treatment method vs. conventional purification method