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. Author manuscript; available in PMC: 2025 Sep 9.
Published in final edited form as: Methods Mol Biol. 2023;2652:147–169. doi: 10.1007/978-1-0716-3147-8_8

Fig. 4.

Fig. 4

(a) SDS-PAGE of purification of PBD46 (5.1 kDa) using the heat treatment method. Lane-1, MWM; lane-2, PBD46 bound to GST; lane-3, PBD46 after cleavage by thrombin; lane-4, 30 °C treatment pellet; lane-5, 30 °C treatment supernatant; lane-6, 50 °C treatment pellet; lane-7, 50 °C treatment supernatant; lane-8, 70 °C treatment pellet; lane-9, 70 °C treatment supernatant; lane-10, 90 °C treatment pellet; lane-11, 90 °C treatment supernatant. (b) DSC spectrum of PBD46 purified by heat treatment. The Tm of the peptide is 41.6 °C (black) which was consistent with the observed Tm of 41.3 °C for PBD46 produced by the conventional double chromatography method (gray). (c) Mass spectrum confirming the size of PBD46. The mass is around 10 kDa because PBD46 is known to dimerize. (d) MALDI-TOF spectrum of PBD46 was analyzed to confirm the amino acid sequence of the peptide