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. Author manuscript; available in PMC: 2025 Sep 9.
Published in final edited form as: Methods Mol Biol. 2023;2652:147–169. doi: 10.1007/978-1-0716-3147-8_8

Fig. 6.

Fig. 6

(a) SDS-PAGE of purification of WAP (7 kDa): lane-1, pellet after lysis; lane-2, supernatant after lysis; lane-3, eluted GST-WAP; lane-4, cleaved GST WAP; lane-5, pre-stained protein marker; lane-6, WAP. (b) Mass spectrum confirms the size of WAP. (c) SDS-PAGE of purification of the HB-peptide (3.7 kDa): lane-1, pellet after lysis; lane-2, supernatant after lysis; lane-3, flow through; lane-4, eluted GST-HB; lane-5, cleaved GST HB; lane-6, pellet after heat treatment; lane-7, HB-peptide; lane-8, pre-stained protein marker. (d) Mass spectrum confirms the size of HB-peptide