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. Author manuscript; available in PMC: 2025 Sep 9.
Published in final edited form as: Methods Mol Biol. 2023;2652:147–169. doi: 10.1007/978-1-0716-3147-8_8

Table 1.

Conditions to prepare the separating and stacking gel solutions

Materials Resolving gel Stacking gel
Total volume 8 mL 5 mL
ddH2O 2.6 mL 2.6 mL
30% acrylamide 3.2 mL 1 mL
0.5 M Tris-HCl pH 8.8 2 mL -
0.5 M Tris-HCl pH 6.8 - 1.25 mL
10% SDS 80 μL 50 μL
10% APS 80 μL 50 μL
TEMED 8 μL 8 μL