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Packaging of Ykt6p into COPII-coated vesicles. COPII budding reactions with semi-intact cells from WT (CBY740) and sec22Δ (CBY773) strains. One-tenth of a total reaction (T), budded vesicles isolated after incubation with COPII proteins (+), or a mock reaction without COPII proteins (−). Samples were resolved by SDS-PAGE, followed by immunoblot for Sec61p (ER resident protein), Erv25p (vesicle protein), and Ykt6p.
