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. 2002 Sep;13(9):3314–3324. doi: 10.1091/mbc.E02-04-0204

Figure 5.

Figure 5

Incorporation of Ykt6p into ER-Golgi SNARE complexes. Solubilized proteins were bound to anti-Bos1p coupled to protein A beads (+) or beads alone (−) as described in MATERIAL AND METHODS. (A) Semi-intact cells from WT (CBY740) and sec22Δ (CBY773) strains were preincubated at 25 or 35°C and then placed on ice before solubilization with Triton X-100. Total solubilized extracts (T) and immunoprecipitates (IP) were resolved on SDS-PAGE followed by immunoblot for indicated proteins. (B) Semi-intact cells from WT (CBY740), WT+pYKT6-2 μm (CBY1108), and sec22Δ (CBY773) strains were preincubated at 25°C, solubilized, and immunoprecipitated with anti-Bos1p–coupled protein A beads as described above.