Figure 3.

Treatment of CHO-mLRP4 cells with proteasomal inhibitors increases the steady state levels of mLRP4. (A) CHO-mLRP4 cells were treated with either vehicle DMSO or DMSO containing MG132 (20 μM) for various periods of time. Equal quantities of cell lysates were separated via SDS gel (6%) and blotted with anti-HA antibody. Only the precursor form and the ligand-binding subunit of the mature form are detected in the blot because these forms, but not the 85-kDa subunit mature form, contain the HA epitope. Note the increase of the mature form, but not the precursor form of mLRP4 after MG132 treatment. (B) The effects of proteasomal inhibition on steady state levels of mLRP4 were also seen with lactacystin, but not with vehicle DMSO alone.