Figure 7.
MPR46 has a shorted half-life and altered subcellular localization in LAMP-2–deficient hepatocytes. (A) Western blotting of MPR46 from control (+/+) and LAMP-2 −/− cells. MPR46−/− and MPR300 −/− hepatocytes are shown as controls. (B) Northern blotting of MPR46 and actin. (C) Immunoprecipitation of MPR46. The cells were labeled with [35S]methionine for 12 h. Leupeptin (100 μM) and pepstatin (100 μM) were added to the chase medium as indicated (Leu/Pep). Quantitation of the bands is shown at the bottom. (D and E) Immunofluorescence of MPR46 from control (D) and LAMP-2–deficient cells (E).