Table 2. PCR premix for IgH, IgK and IgL amplification.

Component	Per well (µL)	One plate (µL)	Final concentration
Water	7.45	745	-
Phanta buffer (2×)	10	1000	-
dNTP (10 mmol/L)	0.4	40	0.2 mmol/L
1mFH/K/L mix (50 µmol/L)	0.15	15	0.38 µmol/L
1mRG/K/L (50 µmol/L)	0.1	10	0.25 µmol/L
Phanta polymerase	0.4	40	-
Total	18.5	1850	-