Table 3. Thermal cycling program for IgH, IgK and IgL amplification.

Step	Temperature	Time	Cycles
Initial denaturation	95°C	30 s	1
Denaturation	95°C	15 s	50
Annealing	46°C	15 s	50
Extension	72°C	1 min	50
Final extension	72°C	10 min	1
Hold	16°C	∞	-