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| Component | Per well (µL) | One plate(µL) | Final concentration |
| Water | 7.64 | 764 | - |
| 2× Es Taq MasterMix | 10 | 1000 | - |
| 2mFG/K or 2mFL mix (50 µmol/L) | 0.18 | 18 | 0.45 µmol/L |
| 2mRG/K/L (50 µmol/L) | 0.18 | 18 | 0.45 µmol/L |
| Total | 18 | 1800 | - |
