Figure 2.

Expression of HA tagged CFAP410 variants

(A) Western blot of two representative clones from each genotype immunoblotted using anti-HA antibody.

(B) Quantified expression levels of CFAP410-HA normalized to GAPDH loading control from three replicate western blots (Data are represented as mean ± SEM).

(C) Immunostaining for PCNT and HA in representative clones counterstained with DAPI. Inset shows a close crop of centriole and basal body. Scale bar 5 μm. Equivalent images for the heterozygous clones can be found in Figure S2.

(D) Co-immunoprecipitation using an antibody against the HA tag and immunoblotting for NEK1, SPATA7 and FBXO3. Performed on neuronal culture lysates of R1 (CFAP410^WT/WT), G24 and H13 (CFAP410^WT-HA/WT-HA), C15 and F02 (CFAP410^{V20M-HA/V20M-HA}), D18 and J13 (CFAP410^{R73P-HA/R73P-HA}). Quantification of immunoprecipitated HA tagged CFAP410 (E) and co-immunoprecipitated NEK1 (F), SPATA7 (G), and FBXO3 (H) from three replicate western blots (Data are represented as mean ± SEM). NEK1, SPATA7, and FBXO3 normalized to HA. Data compared by ANOVA with Bonferroni’s post hoc test. ∗p < 0.05.