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. 2025 Jul 15;26(17):4219–4247. doi: 10.1038/s44319-025-00523-4

Figure EV4. Interaction of borealin with the nucleosome acidic patch.

Figure EV4

(A) Cryo-EM density for the borealin N-terminus bound to a nucleosome showing histone H2A (red), H2B (yellow), H3 (green), H4 (cyan), DNA (blue), and borealin (grey/white), with (B) a colour-coded local resolution map (see inset scale 2–6 Å). PDB ID 8RUQ, EMDB ID - EMD-19514, Table EV1A (all particles). (C) Top (left) and side (right) views of the fitted model are shown. (D) Gold standard Fourier shell correlation (GSFSC) resolution, blue horizontal line indicates an FSC value of 0.143, and (E) particle orientation distribution within the final map.