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. Author manuscript; available in PMC: 2005 Oct 5.
Published in final edited form as: Cell Tissue Bank. 2005;6(1):45–54. doi: 10.1007/s10561-005-5810-0

Figure 1.

Figure 1

Effect of 2D (monolayer) or 3D (porous collagen sponge) conditions on phenotype of bovine articular chondrocytes cultured for 1, 2, and 4 weeks. (a) Total sulfated glycosaminoglycan content (s-GAG) was measured by DMB assay and normalized to DNA content in each dish or sponge (n = 5). (b) Ratio of collagen type II (COL II) to collagen type I (COL I) was calculated from levels of each RNA as measured by quantitative, competitive RT-PCR assays and normalized to glyceraldehyde 3-phosphate dehydrogenase (G3PDH). (c) Ratio of aggrecan (AGG) to collagen type I (COL I) was calculated from levels of each RNA as measured by quantitative, competitive RT-PCR and normalized to G3PDH.