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. Author manuscript; available in PMC: 2005 Oct 5.
Published in final edited form as: Cell Tissue Bank. 2005;6(1):45–54. doi: 10.1007/s10561-005-5810-0

Figure 2.

Figure 2

Photomicrographs of bovine articular chondrocytes cultured in collagen sponges (Toluidine blue stain). Matrix that contains sulfated glycosaminoglycans appears pink/purple and cell nuclei are blue. (a) Collagen sponge was precultured for 1 week in medium containing FBS. Scale bar is 50 μm. (b) Collagen sponge was cultured in FBS for an additional week. (c) After preculture, collagen sponge was cultured in media containing 2% Nutridoma for 7 days. (d) After preculture, collagen sponge was cultured in media containing ITS+3 for 7 days. Arrows indicate pycnotic cells.