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. 2005 Aug 23;7(5):R844–R853. doi: 10.1186/bcr1312

Figure 4.

Figure 4

CystC antagonizes TGF-β signaling in human MCF10A-CA1a breast cancer cells. (a) Control and CystC-expressing MCF10A-CA1a cells were cultured in soft agar for 14 days, whereupon MCF10A-CA1a colony formation was quantified by light microscopy. Values are colony formation per microscope field (means ± SEM) observed in two independent experiments. CystC expression significantly reduced anchorage-independent growth of MCF10A-CA1a cells (*P < 0.05; Student's t-test). (b) Control and CystC-expressing MCF10A-CA1a cells were transiently transfected with p3TP-luciferase and pCMV-β-Gal cDNAs, and were subsequently stimulated with transforming growth factor-β1 (TGF-β1; 0.5 ng/ml) for 24 hours. Afterward, luciferase and β-Gal activities contained in detergent-solubilized cell extracts were measured. Values are luciferase activities (means ± SEM) observed in two independent experiments normalized to the maximal reporter gene expression stimulated by TGF-β in cells expressing green fluorescent protein. CystC expression significantly inhibited luciferase expression induced by TGF-β (*P < 0.05; Student's t-test).