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Effect of TNF-α on percentage of apoptotic somatotropes from rats killed at selected stages of the estrous cycle. Cells from rats killed at diestrus or proestrus were incubated in the presence of TNF-α (50 ng/ml). Each column represents the percentage ± CLs of TUNEL-positive somatotropes (n > 1500 cells from three to four separate experiments, each one performed with three rats per group). **, P < 0.01 vs. respective control without TNF-α.
