Fig. 3. Gel filtration chromatography of LeuRS and ProRS.

LeuRS and ProRS were applied either alone or combined after preincubation to a Superose 12 column equilibrated in low (A) or high (B) salt buffer. Sample chromatograms (A_{280 nm}) for LeuRS (dotted line), ProRS (dashed line), and LeuRS·ProRS (solid line) at low salt (V₀ = 7.0 ml) and high salt (V₀ = 7.3 ml) are shown. Calibration curves (inset) show the elution positions of both individual and combined proteins.