Table II. Yeast two-hybrid interaction between LeuRS and ProRS.

Growth phenotypes on selective media for interactions between reference proteins (A–D are controls (ProQuest two-hybrid system; Invitrogen)) and between ProRS (encoded in the bait vector pDBleu-proS) and library inserts (encoded in the prey vector pDEST) are shown. Growth of transformed MaV203 after 48 h at 30 °C on SC lacking leucine and tryptophan supplemented as indicated is shown. −, no growth; +, weak growth; ++, moderate growth; +++, strong growth.

Plasmids	−Ura	−His, +25 mm 3-AT	+0.2% 5-fluoroorotic acid	Description
A controls	−	-	++	No Interaction standard
B controls	+/−	+	+	Weak Interaction standard
C controls	++	+	+/−	Moderate interaction standard
D controls	+++	+++	++	Strong interaction standard
pDBleu-proS/pDEST-I149	−	+	+++	Glutaminase insert in prey vector
pDBleu-proS/pDEST-I150	+/−	+++	+++	LeuRS insert in prey vector
pDBleu-proS/+ pPC86	−	+/−	+++	Empty prey vector control