Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady-state aminoacylation kinetics of M. thermautotrophicus ProRS
| Enzymea | Additionsb | Km Pro | Km tRNAPro | kcat | kcat/Km tRNA (relative) |
|---|---|---|---|---|---|
| μm | μm | s−1 | |||
| ProRS | None | NDc | 4.1 ± 0.9 | 1.8 ± 0.2 | 1 |
| ProRS | LeuRS | ND | 2.2 ± 0.6 | 5.1 ± 0.4 | 5 |
| ProRS | BSA | 67 ± 10 | 2.4 ± 0.4 | 2.9 ± 0.1 | 3 |
| ProRS | LeuRS, BSA | 61 ± 17 | 1.4 ± 0.1 | 6.9 ± 0.2 | 12 |
Enzymes were added at a final concentration of 40 nm.
Other components at 400 nm each.
ND, not determined.
