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Steady-state aminoacylation kinetics of M. thermautotrophicus LeuRS
| Enzymea | Additionsb | kcat/Km Leu | kcat/Km tRNALeu |
|---|---|---|---|
| s−1 μm−1 | |||
| LeuRS | BSA | 0.35 ± 0.07 | 0.75 ± 0.08 |
| LeuRS | ProRS, BSA | 0.37 ± 0.09 | 0.75 ± 0.03 |
Enzymes were added at a final concentration of 40 nm.
Other components at 400 nm each. Due to the high Km compared to practical tRNA concentrations ([S] ≪ Km), kcat/Km was directly estimated from the equation v = kcat / Km ([E] [S]).
