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. Author manuscript; available in PMC: 2005 Oct 5.
Published in final edited form as: Mol Endocrinol. 2005 Apr 28;19(8):2132–2144. doi: 10.1210/me.2004-0472

Fig. 6. The Presence of the Seventh TMD and the C-Terminal Tail of the CTR Is Not Necessary for Dimerization of the CTR.

Fig. 6

HEK 293 cells were transfected with the C1a-FLAG cDNA alone or in combination with GFP-tagged C1a, Δe13, C1aΔ397, or C1aΔ374, as indicated. Thirty-six to 48 h after transfection, the cells were lysed and the CTR-GFP construct was immunoprecipitated (IP) using the monoclonal anti-GFP antibody. The immune complexes were immunoblotted (IB) with the anti-FLAG antibody (upper panel). The membrane was stripped and reblotted with the anti-GFP antibody (middle panel). To show equal amounts of the FLAG-tagged CTR construct in the different lysates, total cell lysates (TCL) were immunoblotted with the anti-FLAG antibody (lower panel). Identical results were obtained in three independent experiments.